Whilst we haven't analyzed PneumaCult™ media for use with murine epithelial cells, you can find publications that report this software.
The following example is for preparing RNP complexes for one reaction. Change accordingly dependant on the amount of reactions demanded.
Every one of such cells has a task to Engage in. Investigation on how they operate and their interactions with other subsets carries on to reveal possibilities for novel therapeutic approaches.
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Even though we have not directly tested nasal epithelial cells in-dwelling, there are actually publications that report PneumaCult™ supporting this cell style.
Consider the results of take a look at compounds on hematopoietic stem cells with phenotypic and purposeful assessments.
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Indeed, you’ll locate the stage-by-move protocol for TEER measurement to evaluate the epithelial barrier integrity in ALI cultures listed here.
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